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Study of Antimicrobial Compounds of Bacillus subtilis (PSB5) and its Interaction with Fungicides against Fusarium oxysporum f. sp. gerberae

Affiliations

  • Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore - 641003, Tamil Nadu, India
  • Department of Plant Pathology, Agricultural College, Naira-532185, Acharya N. G. Ranga Agricultural University, Andhra Pradesh, India

Abstract


Objectives: To study the antifungal activity of Bacillus spp. against Fusarium oxysporum f. sp. gerberae (FOG) and studies of interaction between Bacillus subtilis (PSB5) and commercially active fungicides against FOG. Methods: Extraction of crude antibiotics (PSB5, BSD5, BmTNAU1) was done by continuous precipitation and acidification to pH 2.0 using solvent ethyl acetate and further subjected to GC/MS analysis (PSB5). Bioassay of these extract against the pathogen (FOG) was done by Agar Diffusion method. The control employed was ethyl acetate alone in the media. Further the compatibility test was done by Poisoned food technique. Findings: The crude antibiotic extracts (PSB5, BSD5, BmTNAU1) were highly effective against the FOG probably due to the presence of antimicrobial compounds in strain PSB5 detected through GC/MS. Moreover, the test of compatibility of PSB5 with fungicides revealed the growth promotion of the bacteria at lower dosages of fungicides alone. The most effective fungicide, tebuconazole 250 EC(Score) against FOG showed compatibility at moderate dosage (250 ppm) with the highly efficient strain of bacteria, PSB5. Application: In our present study, we tried to emphasize on the efficacy of antifungal activity of Bacillus spp. and compatibility interactions of antagonistic bacteria and fungicides which could be utilized for further field experiments against FOG.

Keywords

Antimicrobial Compounds, Bacillus, Fungicides, Fusarium, Gerbera.

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References


  • Sujatha K, Gowda JVN, Khan MM. Effects of different fertigation levels on Gerbera under low cost greenhouse. Journal of Ornamental Horticulture. 2002; 51(1):54–9.
  • Nagrale DT, Gaikwad AP, Goswami S, Sharma L. Fungicides management of Alternaria alternata (Fr.) Keissler causing blight of Gerbera (Gerbera jamesonii H. Bolus ex J. D. Hook). Journal of Applied and Natural Science. 2012; 4(2):220–7.
  • Silo-suh LA, Lethbridge BJ, Raffel SJ, He H, Clardy J, Handelsman J. Biological activities of two fungistatic antibiotics produced by Bacillus cereus UW Applied. Environonmental Microbiology. 1994; 60(85):2023–30.
  • Wei WLIU, Wei MU, Bing-yu ZHU, You-chenDU, Feng LIU. Antagonistic activities of volatiles from four strains of Bacillus spp. and Paenibacillus spp. against Soil-Borne Plant Pathogens. Agricultural Sciences in China. 2008; 7(9):1104–14.
  • Dean M, Guerrini P, Paci F, Brunelli A, Canova A, Collina M. Compatibility of the antagonistic bacterium Bacillus subtilis BSF4 with fungicides and insecticides. Giornate Phytopathologiche; 2006. p. 437–42.
  • Tutte J. Plant pathological methods Fungi and bacteria. Burgess Publishing Company, USA; 1969. p. 229.
  • Burgess LW, Nelson PE, Toussoun TA, Forbes GA. Distribution of Fusarium species in sections Roseum, Arthrosporiella, Gibbosum and Discolor recovered from grassland, pasture and pine nursery soils of eastern Australia. Mycologia. 1989; 80(2):815–24.
  • Wratten SJ, Wolfe MS, Anderson RJ, Faulkner DJ. Antibiotic metabolites from the marine Pseudomonas. Antimicrobiological Agents and Chemotherapy. 1977; 11(3):411–14.
  • Tagg JR, McGiven AR. Assay system for bacteriocins. Applied Microbiology. 1971; 21(5):943.
  • Grover RK, Moore JD. Toxicometric studies of fungicides against brown rot organisms Sclerotinia fructicola and S. laxa. Phytopathology.1962; 52(2):876–80.
  • Garibaldi A, Minuto A, Bertetti D, Gullino ML. Fusarium Wilt of Gerbera in Soil and Soilless Crops in Italy. Plant Disease. 2004; 88(3):311.
  • Garibaldi A, Minuto A. Fusarium wilt of gerbera in Spain in soilless crops. Plant Disease. 2007; 91(5):638.
  • Booth C. The genus Fusarium. Common wealth Mycological Institute, Kew Surrey, UK. 1971; 64(4):142–3.
  • Zhang SM, Wang YX, Meng LQ, Li J, Zhao XY. Isolation and characterization of antifungal lipopeptides produced by endophytic Bacillus amyloliquefaciens TF28. African Journal of Microbiological Research. 2011; 6(8):1747–55.
  • Islam RM, Jeong YT, Lee SY, Song HC. Isolation and identification of antifungal compounds from Bacillus subtilis C9 inhibiting the growth of plant pathogenic fungi. Mycobiology. 2012; 40(1):59–66.
  • Wang H, Yan HY, Wang JM, Zhang PH, Qi W. Production and Characterization of Antifungal Compounds Produced by Lactobacillus plantarum IMAU10014. PLoS ONE. 2012; 7(1):1371.
  • Ahmad B, Khan I, Bashir S, Azam S. Chemical composition and antifungal, phytotoxic, brine shrimp cytotoxicity, insecticidal and antibacterial activities of the essential oils of Acacia modesta. Journal of Medicinal Plants Research. 2012; 6(31):4653–9.
  • Salem MZM, Ali HM, Mansour MM. Fatty acids methyl esters from air dried wood, bark and leaves of Brachichyton diversifolius R.Br: Antifungal, Antibacterial and Antioxidant activities. Bioresources. 2014; 9(3):3835–45.
  • Kumar SRS. GC- MS analysis and antibacterial potential of white crystalline solid from red algae Portieria hornemannii against the plant pathogenic bacteria Xanthomnas axonopodis pv. citri (Hasse) and Xanthomonas campestris pv. malvacearum (smith 1901) dye 1978b. International Journal of Advanced Research. 2014; 2(3):174–83.
  • Vijayalakshmi K, Premalatha A, Rajakumar GS. Antimicrobial protein production by bacillus amyloliquefaciens MBL27: Optimization of Culture Conditions Using Taguchi's Experimental Design. Indian Journal of Science and Technology. 2011; 4(8):931–7.
  • Suneeta P, Eraivan AAK, Nakkeeran S. In vitro management of Fusarium wilt of Gerbera by novel Trichoderma spp. and fungicidal molecules. International Journal of Agricultural Science and Research. 2016; 6(5):327–32.
  • Pallavi RV, Nepolean P, Balamurugan A, Jayanthi R, Beulah T, Premkumar R. In vitro studies of biocontrol agents and fungicides tolerance against grey blight disease in tea. Asian Pacific Journal of Tropical Biomedicine. 2012; 2(1):435–8.

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