Total views : 212

Revisiting the Issue of the Molecular-genetic Structure of the Causative Agent of the Bovine Leukemia Virus in the Russian Federation


  • Ural State Agrarian University, Yekaterinburg, Sverdlovsk region, Russian Federation
  • Ural Scientific Research Veterinary Institute, Yekaterinburg, Sverdlovsk region, Russian Federation
  • Department of Biochemistry, National Veterinary Research Institute, Pulawy, Poland


Objectives: The study is aimed at investigating the genotypic diversity of the causative agent of Bovine Leukemia, circulating in animal populations from different regions of Russia. Methods: Genotyping and sequencing for samples of 444 bp gene DNA element of cattle HVL isolates of Russian regions were conducted and phylogenetic analysis of the isolated strains was performed. Findings: Blood samples, serum and leukoconcentrate of animal blood and DNA samples – env gene region, 444bp long, of cattle leukemia virus were a subject of investigation. Samples from Belgian subgroup (type B) and Australian subgroups (type A) of leukemia virus were singled out in the studied samples. The results of this study provide an opportunity to study features of leukemic epizootic process in the region. For the first time in the middle Urals in Russia the molecular-genetic structure of the cattle leukemia virus was singled out. Phylogenetic analysis of virus derived from DNA sequencing has allowed classifying the studied strains and comparing them with global isolates. Determination of the virus genotype allows for deeper research to identify the pathogenetic variants of leukemic process development in a particular region – in the middle Urals. Study of genotypic diversity of the cattle leukemia virus opens new opportunities and additional approaches to the development of methods for health improvement in leukemia disadvantaged populations. Applications/Improvements: The data obtained will serve as a base for the creation of highly sensitive, specific primers for effective PCR diagnostics.


Cattle, Chain Reaction Method, DNA-sequencing, Genotype, Leukemia Virus, Molecular-genetic Structure, Polymerase.

Full Text:

 |  (PDF views: 166)


  • Donnik IM, Petropavlovskiy VM, Tatarchuk AT et al. Sensitivity and specificity of diagnostic tests (ELISA, PCR) in the detection of virus carriers among cattle VL heals from leucosis in cattle. In: Modern problems of diagnostics, treatment and prevention of diseases of animals and birds: Collection of scientific works of leading scientists of Russia and Abroad. Vol. 3. Yekaterinburg: Ural Publishing House; 2010. p. 113–18.
  • Gulyukin MI, Velikhov AF, Nakhmanson VM, Ivanov AL, Grek KP, Lapunov SV. Peculiarities of infectious process induced by leukemia virus in cattle. Proceedings of International Scientific-Practical Conference dedicated to the 110th Anniversary of All-Russian Scientific Research Institute of Experimental Veterinary. Moscow; 2008. p. 106–13.
  • Gladyr EA, Zinovieva NA, Ermilov AA, Vinogradov VN, Ernst LK. Development and pilot testing of a highly sensitive test-system for diagnostics of leukemia virus of cattle. Veterinary Pathology. 2006; 3:87–9.
  • Beier D, Blankenstein P, Marquardt O, Kuzmak J. Identification of different BLV provirus isolates by PCR, and DNA sequencing RFLPA. Berliner und Münchener tierärztliche Wochenschrift. 2001 Jul–Aug; 114(7–8):252–6.
  • Rodriguez SM, Golemba MD, Campos RH, Trono K, Jones LR. Bovine leukemia virus can be classified into seven genotypes: evidence for the existence of two novel clades. Journal of General Virology. 2009, Nov; 90(Pt 11):2788–97.
  • Gulyukin MI, Lomakin NF, Kozyrev NG, Ivanov LA, Klimenko AI, Kovalenko AV. The variability of gene env proviral leukemia cattle circulating in the Rostov Region. Proceedings of All-Russian Scientific-Practical Conference of GNU SKZNIVI RAAS – Novocherkassk; 2009. p. 22–6.
  • Rice NR, Stephens RM, Couez D, Deschamps J, Keltmann R, Burny A, Gilden RV. The nucleotide sequence of the env gene and post-env region of bovine leukemia virus. Virology. 1984; 138(1):82–93.
  • Sagata N, Yasunaga T, Tsuziiku-Kawnmurn J, Ohishi K, Ogawa Y, Ikawa Y. Complete nucleotide sequence of the genome of bovine leukemia virus: its evolutionary relationship to other retro-viruses. Proceedings of the National Academy of Sciences. 1985; 82(3):677–81.
  • Drobot EV. The results of the study of genotypic diversity of the bovine leukemia virus, and epidemiological features and haematological manifestations of leukemia. Author’s abstract of PhD Thesis in Biology. Novosibirsk; 2007.
  • Gracheva NI, Smirnov PN. The incidence of leukemia and the milk yield of ilirski breed cows of different genealogical lines. Proceedings of International Scientific-Practical Conference Adaptation, Health and Productivity of Animals, Ekaterinburg, 2008 May 22–23; 2008. p.76–8.
  • Mamoun RZ, Morisson M, Rebeyrotter N, Busetta B, Couez D, Kettmann R, Hospital M, Guilemain B. Sequence variability of bovine leukemia virus env gene and its relevance to the structure and antigenicity of glycoproteins. Journal of Virology. 1990; 64(9):4180–8.
  • Fechner H, Kurg A, Blankenstein P, Mewes G, Geue L, Albrecht C, Ebner D. Direct use of cell lysates in PCR-based diagnosis of bovine leukemia virus infection. Berliner und Münchener tierärztliche Wochenschrift. 1996; 109(11–12):446–50.
  • Licursi M, Inoshima Y, Wu D, Yokoyama T, Gonzalez ET, Sentsui H. Genetic heterogeneity among bovine leukemia virus genotypes and its relation to humoral responses in hosts. Virus Research. 2002, 86(1–2):101–10.
  • Asfaw Y, Tsuduku S, Konishi M, Murakami K, Tsuboi T, Wu D, Sentsui H. Distribution and superinfection of bovine leukemia virus genotypes in Japan. Archives of Virology. 2005; 150(3):493–505.
  • Moratorio G, Obal G, Dubra A, Correa A, Bianchi S, Buschiazzo A. Cristina J, Pritsch O. Phylogenetic analysis of bovine leukemia viruses isolated in South America reveals diversification in seven distinct genotypes. Archives of Virology. 2010; 155(4):481–9. DOI: 10.1007/s00705-010-0606-3.
  • Zinovieva NA, Gladyr EA, Bykova AS. Modeling screening systems BOS TAURUS on genetic resistance to bovine leukemia virus. In: current achievements and challenges of agricultural animal biotechnology: the role of nanotechnology in the implementation of the priority tasks of biotechnology 7th international scientific conference-school; 2008. p. 33–40.
  • Portetelle D, Dandoy C, Burny A, Zavada J, Siakkou H, Gras-Masse H, Drobecq H, Tartar A. Synthetic peptides approach to identification of epitopes on bovine leukemia virus envelope glycoprotein gp51. Virology. 1989; 169(1):34–41. DOI: 10.1016/0042-6822(89)90038-X.
  • Beier D, Blankenstein P, Marquardt O, Kuzmak J. Identification of different BLV provirus isolates by PCR, and DNA sequencing RFLPA. Berliner und Münchener tierärztliche Wochenschrift. 2001; 114(7–8):252–6.
  • Coulston J, Naif H, Brandon R, Kumar S, Khan S, Daniek RC, Lavin MF. Molecular cloning and sequencing of an Australian isolate of proviral bovine leukaemia virus DNA: comparison with other isolates. Journal of General Virology. 1990; 71(8):1737–46. DOI: 10.1099/0022-1317-71-8-1737.
  • Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Molecular Biology and Evolution. 2007; 24(8):1596–9. DOI: 10.1093/molbev/msm092.
  • NCBI. National Center for Biotechnology Information [Internet]. Available from:
  • Petropavlovskiy MV. Effectiveness of diagnostic tests in the detection of the cattle leukemia virus in heals from leukemia herds. Author’s abstract of PhD Thesis in Veterinary. Ekaterinburg; 2010.
  • Matsumura K, Inoue E, Osawa Y, Okazaki K. Molecular epidemiology of bovine leukemia virus associated with enzootic bovine leukosis in Japan. Virus Research. 2011; 155(1):343–48. DOI: 10.1016/j.virusres.2010.11.005.
  • Bykova AS, Zinovieva NA, Gladyr EA. Rapid diagnosis of BOLA-DRB3alleles associated with cattle resistance to leukemia. Problems of Biology of Productive Animals. 2011; 1:12–15.
  • Vinogradova IV, Gladyr EA, Zinovieva NA. Comparative evaluation of the effectiveness of REID and DNA-diagnostics of leukemia virus of cattle. Problems of Biology of Productive Animals. 2011; 1:15–16.
  • Kuzmak J, Rola M, Kozaczynska B, Pluta A. Bovine Immunodeficiency Virus as a potentiating cofactor for the experimental Bovine Leukemia Virus infection in sheep. Retrovirology. 2011 Jun; 8(Suppl 1):1. DOI: 10.1186/1742-4690-8-S1-A5.
  • Donnik IM. System of measures of rehabilitation of cattle from leukemia in the Urals Federal district. Donnik IM, Smirnov PN, Sivkov GS, Shevkoplyas VN, Shkuratova IA, Tatarchuk AT, editors. Scientific advice, Ekaterinburg: Ural publishing house; 2008. p. 66.
  • Donnik IM. Regional molecular genetic structure of the cattle leukemia virus / IM Clover, Tatarchuk AT, Lysov AV, Mikheev PM. Veterinary Kuban. – Krasnodar. 2010; 3:5–6.


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.