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Optimization of Electric Field Parameters for HT29 Cell Line towards Wound Healing Application
Objective: The aim of this study is to optimize the electric field parameter for HT-29 cells line towards wound healing application. Methods: Cells were harvested when they reached 70% to 80% confluent. Afterward, 800 μl of the cells suspension were poured into a 4 mm cuvette and placed in the BTX ECM 830 Electroporator chamber. Voltages in the range of 80 V to 800 V and pulse durations in the range of 100 μs to 10 ms were used in electroporating the cells. After treatment, 300 μl of the cells suspension from each sample were seeded in a well of 6-wells plates each containing 2 ml of pre-warm complete growth medium and incubated at 37oC and 5% CO2 for 48 hours. Findings: The results obtained in this research quantitatively reveal the dependence of cell proliferation on electrical parameters. 200, 400, 600 and 800 V/cm electric field strengths at duration of 500 μs showed 42%, 63%, 86% and 36% increase in proliferation rate after 48 hours in culture as compared to initial plating densities, respectively. The control group and 1000 V/cm at 100 μs showed 48% and 33% increase in proliferation rate respectively as compared to initial seeding density. While electric field strengths of 1200-2000 V/cm at 200 V/cm interval and at 100 μs duration, revealed a decrease in proliferation rate by 4%, 15%, 18%, 23% and 26% respectively as compared to initial seeding density. Applications: It was found that 600 V/cm at 500 μs induced the highest proliferation rate (86%) as compared to initial seeding density after 48 hours of culture (that is 38% greater than the control group). This study can further be investigated for the use of the pulse electric field in facilitating drug-free wound healing process.
Electroporation, Electric Field, Optimization, Proliferation, Wound Healing.
- Zupanic A. Treatment planning in biomedical applications of electroporation. [Ph.D thesis]. Slovenia: University of Ljubljana; 2010. p. 224.
- Pucihar G, Krmelj J, Reberssek M, Napotnik T, Miklavcicc D. Equivalent pulse parameters for electroporation. IEEE Transactions on Biomedical Engineering. 2011 Sep; 58(11):3279–88.
- Venslauskas M, Satkauskas S, Rodaite R. Efficacy of the delivery of small charged molecules into cells in vitro.Bioelectrochemistry. 2010 Oct; 79(1):130–5.
- Krassowska W, Nanda G, Austin M, Dev S, Rabussay D.Viability of cancer cells exposed to pulsed electric fields: The role of pulse charge. Ann Biomed Eng. 2003 Jan; 31(1):80–90.
- Canatella J, Karr J, Petros J, Prausnitz M. Quantitative study of electroporation-mediated molecular uptake and cell viability.Biophys J. 2001 Feb; 80(2):755–64.
- Lye HS, Khoo BY, Karim AH, Rusul G, Liong MT. Growth properties and cholesterol removal ability of electroporated Lactobacillus acidophilus BT 1088. Journal of Microbiology and Biotechnology. 2012 Mar; 22(7):981–9.
- Mamman H, Abdul Jamil M. Investigation of electroporation effect on HT29 cell lines adhesion properties. 2015 IEEE 2nd International Conference on Biomedical Engineering (ICoBE); 2015 Mar 30-31. p. 1–5.
- Mamman H, Sadiq A, Adon M, Abdul Jamil M. Study of electroporation effect on HT29 cell migration properties.2015 IEEE International Conference on Control System, Computing, and Engineering (ICCSCE 2015); 2015 Nov 27-29. p. 342–6.
- Rebersek M, Miklavcic D. Advantages and disadvantages of different concepts of electroporation pulse generation.Journal Automatika. 2011; 52(1):12–9.
- Tryfona T, Bustard M. Impact of pulsed electric fields on Corynebacterium glutamicum cell membrane permeabilization.Journal of Bioscience and Bioengineering. 2008 Jan; 105(4):375–82.
- Dubey A, Gupta S, Basu B. Optimization of electrical stimulation parameters for enhanced cell proliferation on Biomaterial surfaces. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2011 Feb; 98B(1):18–29.
- Pehlivanova V, Tsoneva I, Tzoneva R. Influence of electroporation on cell adhesion, growth and viability of cancer cells and fibroblasts. Biologie Cellulaire. 2010 Dec; 64(4):581–90.
- Saulis G, Lape R, Praneviciute R, Mickevicius D. Changes of the solution pH due to exposure by high-voltage electric pulses. Bioelectrochemistry. 2005 Sep; 67(1):101–8.
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