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Silk Protein based Novel Matrix for Tissue Engineering Applications
Tissue engineering deals with recreating, maintaining and enhancing the damaged or lost tissue/organ of living organisms. For tissue engineering applications, biomaterials are needed, that can be derived from either natural or synthetic source. Silk is one of the best natural biomaterials because of its properties like biocompatibility, high mechanical strength and slow degradation rate in vivo. Present study describes the synthesis of silk fibroin hydrogel using photopolymerization method. Aqueous silk fibroin solution was blended with synthetic polymer. The solution was then exposed by a monochromatic light source for the polymerization process. During the polymerization process, sample was at a specific distance from the light source under controlled temperature conditions i.e; at 37⁰C. After the incubation period of around 3 hours, matrix with soft, slippery and elastic surface was obtained. The matrix obtained can further be used as a source for drug delivery carrier, for sustained delivery of drug, in the field of gene therapy for controlled release of DNA at the targeting site and also be used for bone and cartilage tissue engineering.
Biocompatibility, Biomaterial, Degradation, Fibroin, Sericin, Silk Proteins, Tissue Engineering
- Rice WL, Firdous S, Gupta S, Hunter M, Foo CWP, Wang Y, Kim HJ, Kaplan DL, Georgakoudi I. Non-invasive characterization of structure and morphology of silk fibroin biomaterials using non-linear microscopy. Biomaterials.2008 February; 29:2015-24. Crossref. PMid:18291520 PMCid:PMC2737252.
- Padamwar MM, Pawar AP. Silk sericin and its applications: A review. Journal of Scientific and Industrial Research. 2004 April; 63:323-9.
- Joseph B, Raj JS. Therapeutic applications and properties of silk proteins from Bombyx mori. Frontiers in Life Science 6. 2012 December; 6:55-60.
- Kearns V, MacIntosh AC, Crawford A, Hatton PV. Silk-based Biomaterials for Tissue Engineering. UK: Topics in Tissue Engineering Publication. 2008; 4.
- Seib FP, Herklotz M, Burke KA, Maitz MF, Werner C, Kaplan DL. Multifunctional silk-heparin biomaterials for vascular tissue engineering applications. Biomaterials. 2014 October; 35:83-91. Crossref. PMid:24099708 PMCid:PMC3872823.
- Kundu B, Kundu SC. Silk sericin/polyacrylamide in situ forming hydrogels for dermal reconstruction. Biomaterials. 2012 July; 33:7456-67.Crossref. PMid:22819495.
- Kapoor S, Kundu SC. Silk protein-based hydrogels: Promising advanced materials for biomedical applications. Acta Biomaterialia. 2015 November; 31:17-32. Crossref. PMid:26602821.
- Cao Y, Wang B. Biodegradation of Silk Biomaterials. International Journal of Molecular Sciences. 2009 March; 10:1514-24. Crossref. PMid:19468322 PMCid:PMC2680630.
- Hu X, Kaplan DL. Silk Biomaterials. Editor-in-Chief: Paul D, editor. Oxford: Elsevier: Comprehensive Biomaterials. 2011; p. 207-19. Crossref.
- Lum L, Elisseeff J. Injectable Hydrogels for Cartilage Tissue Engineering. USA: Topics in Tissue Engineering Publication. 2003.
- Silva R, Fabry B, Boccaccini AR. Fibrous protein-based hydrogels for cell encapsulation. Biomaterials. 2014 May; 35:6727-38. Crossref. PMid:24836951.
- Chirila TV, Suzuki S, McKirdy NC. Further development of silk sericin as a biomaterial: comparative investigation of the procedures for its isolation from Bombyx mori silk cocoons. Progress in Biomaterials. 2016 July; 5:135-45. Crossref. PMid:27525204 PMCid:PMC4965490.
- Sah MK, Kumar A, Pramanik K. The extraction of fibroin protein from Bombyx mori silk cocoon: Optimization of process parameters. International Journal of Bioinformatics Research. 2010; 2:33-41. Crossref.
- Kundu B, Rajkhowa R, Kundu SC, Kundu XWB, Rajkhowa R, Kundu SC, Wang X. Silk fibroin biomaterials for tissue regenerations. Advanced Drug Delivery Reviews. 2012 November; 65:457-70. Crossref.PMid:23137786.
- Kovacina JR, DesRochers TM, Burke KA, Kaplan DL. The effect of sterilization on silk fibroin biomaterial properties. Macromolecular Bioscience. 2015 June;15:861-74. Crossref.
- PMid:25761231 PMCid:PMC4456215.
- Yucel T, Lovett ML, Kaplan DL. Silk-Based Biomaterials for Sustained Drug Delivery. Journal of Control Release. 2014 September; 28:381-97. Crossref. PMid:24910193 PMCid:PMC4142080.
- Sun W, Motta A. Italy: Silk fibroin-based injectable hydrogels for brain tissue engineering applications. 2014.
- Vollrath F. Strength and structure of spiders’ silks. Reviews in Molecular Biotechnology. 2000 April; 74:67-83. Crossref.
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