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Comparative Study of Collagenase and Papain-urea Based Preparations in the Management of Chronic Nonhealing Limb Ulcers


  • Department of General Surgery, M.S.Ramaiah Medical College, Bangalore-560 054, Karnataka, India


The objectives of this study are to compare effectiveness of collagenase v/s papain- urea for debridement of chronic non-healing ulcers/wounds and to evaluate their role in promoting ulcer healing by granulation and reduction in ulcer/wound size. A comparative study of 100 patients was done at M.S. Ramaiah Hospitals in India from November 2007 to August 2009. Patients were selected, randomized, and divided into two groups consisting 50 patients each. Group- 1 treated with collagenase and Group 2 with papain- urea. Patients were evaluated at 0, 1, 2, 3, and 4 weeks for reduction in ulcer size, granulation, discharge and over all response to treatment. The mean age was 42 +/- 15yrs. The co-morbidities were diabetes 28.4 %, hypertension in 21.1%, others 14.0%. Culture and sensitivity test reveals that most frequently grown organism was E. coli (13%) Staphyloccus aureus (9%) and samples with no growth was 64%. In papain-urea group, ulcer was reduced from 24.8 sq. cms to 11.9 sq. cms and collagenase group 23.1 sq. cms to 9.7 sq. cms. There was significant reduction in slough and necrotic tissue i.e. in papain-urea group 22.54 sq. cms to 5.07 sq. cms and collagenase group 21.76 sq. cms to 6.12 sq. cms. Significant amount of increase in granulation tissue in papain-urea group i.e., 2.4 sq. cms to 6.82 sq. cms and collagenase group 1.4 sq. cms to 3.8 sq. cms was observed. But Papain-urea group showed better response in 2nd, 3rd, 4th weeks compared to collagenase (p value <0.05) and significant improvement in 28% in papain-urea, 12% in collagenase group. Mean follow-up period was 7.28- 8.14wks. Papain-urea and collagenase have shown proven efficacy in bringing out enzymatic wound debridement. Papain-urea is a better enzymatic debriding agent promotes faster granulation compared to collagenase.


Collagenase, Papain-urea, Non-healing Ulcer, Enzymatic Debridement, Debridement

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