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Liquid Chromatography Coupled to Mass Spectrometry Based Identification of Elite Chemotypes of Adhatoda vasica Nees for Profitable Agronomy – A Farmer Centric Approach

Affiliations

  • Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam - 515134, Andhra Pradesh, India
  • Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam - 515134, Andhra Pradesh, India

Abstract


In this paper we have identified accessions of a traditional medicinal plant, Adhatoda vasica Nees, from eight ecogeographically distinct nodes of Western Ghats of Malabar area, India. Alkaloids have been extracted from the leaves and analysed qualitatively using spectroscopic techniques. A method has been developed to determine relative amounts of one of the active ingredients – vasicine, using Liquid Chromatography coupled to Mass Spectrometry (LC-MS/MS). The study has revealed samples from Kanjikode and Wadakencherry to have high amounts of vasicine compared to the rest in the study area. Future work should be directed in evaluating samples from similar forests of Western Ghats of Kerala and developing a robust database of metabolite content of various accessions. From such a database, elite chemotypes could be screened and suggested for cultivation by farmers for gaining better income.

Keywords

Adhatoda vasica, Alkaloids, LC-MS, Medicinal Plants, Vasicine, Western Ghats.

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References


  • Harborne AJ. Phytochemical methods: A guide to modern techniques of plant analysis. London: Springer; 1998.
  • Pattnaik SJ, Rao NR, Chary P. Ecomorphometric markers reflect variations in azadirachtin-a content of Azadirachta indica Juss (Meliaceae) in select regions of Andhra Pradesh, India. Current Science. 2006; 91(5):628-36.
  • Raja S, Unnikrishnan K, Satheesh G, Remashree A, Udayan P, Tushar K, Indira B. Variation in vasicine content and pharmacognostic characters of morphotypes of Adhatoda zeylanica Medic. Journal of Plant Sciences. 2008; 3(1):61-8.
  • Raaman N. Phytochemical techniques. New Delhi: New India Publishing House; 2006.
  • Srivastava S, Verma RK, Gupta MM, Singh SC, Kumar S. HPLC determination of vasicine and vasicinone in Adhatoda vasica with photo diode array detection. Journal of Liquid Chromatography Related Technology. 2001; 24(2):153-9.
  • Maviskee R, Holmes H. The alkaloids. New York: Academic Press, Inc.; 1953.
  • Rajpal V. Standardization of botanicals – testing and extraction methods of medicinal herbs. New Delhi: Eastern Publishers; 2005.
  • Ignacimuthu S, Shanmugam N. Antimycobacterial activity of two natural alkaloids vasicine acetate and 2-acetyl benzylamine, isolated from indian shrub Adhatoda vasica ness leaves. Journal of Biosciences. 2010; 35(4):565-70.
  • Shahwar D, Raza M, Tariq S, Riasat M, Ajaib M. Enzyme inhibition antioxidant and antibacterial potential of vasicine isolated from Adhatoda vasica Nees. Pak J Pharm Sci. 2012; 25(3):651-6.
  • Roja G, Vikrant B, Sandur SK, Sharma A, Pushpa K. Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude extracts. Food Chemistry. 2011; 126(3):1033-8.
  • Muralidhar S, Shashidhara S, Rajasekharan P, Hanumantharaju N, Rajendra C. HPLC analysis of Adhatoda vasica obtained from different geographical sources. International Journal of Drug Developoment Research. 2010; 2(4):676-80.

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