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Extremophiles as Biofactories of Novel Antimicrobials and Cytotoxics – An Assessment of Bioactive Properties of Six Fungal Species Inhabiting Rann of Kutch, India


  • Amity Institute of Microbial Technology, Amity University, Noida - 201303, Uttar Pradesh, India
  • PMB Gujarati Science College, Devi Ahilya Vishwavidyalaya, Indore - 452001, Madhya Pradesh, India


Objective: In this study we report the antibacterial and cytotoxic activity of the fungi isolated from Little Rann of Kutch, India. Methods: Antibacterial activity of crude extracts of 35 fungal isolates was assessed at an initial concentration of 40μg/ml against Klebsiella pneumoniae and Escherichia coli. Promising fungal isolates were identified by their morphology. Extracts of identified isolates were further tested at varying concentrations (10-40μg/ml) against Staphylococcus aureus and Bacillus cereus apart from K. pneumoniae and E. coli. Cytotoxic activity was assessed against cancer cell line MCF- 7. Phenol, flavonoid and alkaloid estimation was done to understand the fungi’s chemoprofile. Findings: In preliminary screening, six isolates showed significant activity against K. pneumoniae (14-17 mm) and E. coli (14-22 mm). These were identified as Aspergillus flavus, A. cremeus, A. versicolor, A. terreus, Penicillium purpurogenum and Eurotium amstelodami. On further evaluation of fungal extracts against four test pathogens, A. versicolor was found to be the most effective (8-11 mm), followed by A. terreus (8-10 mm) and A. cremeus (8-10 mm) at 10μg/ml each. A. versicolor and P. purpurogenum were found to be most cytotoxic, with IC50 values of 6.26 and 6.30 respectively, followed by A. flavus, E. amstelodami, A. cremeus and A. terreus. Phenolic content was highest in A. versicolor (268.77±0.18 μg/mg GAE); flavonoid and alkaloid were maximum in P. purpurogenum - 91.17±0.44 μg/mg QE and 89.13±0.28 μg/mg PNE, respectively. Improvements/Applications: Six fungi isolated from Little Rann of Kutch, India, hold tremendous potential as a source of novel antimicrobials and cytotoxics. The results warrant more exhaustive bioassays and identification of active principles.


Antibacterial, Aspergillus, Cytotoxicity, Eurotium, Penicillium, Rann of Kutch.

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