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In Vitro Regeneration of Pigeonpea [Cajanus cajan (L.) Millsp.] Genotype GT 101 using Cotyledonary Node

Affiliations

  • Department of Plant Molecular Biology and Biotechnology, C. P. College of Agriculture, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar-385506, Gujarat, India
  • Department of Biotechnology, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India
  • Shree P. M. Patel Institute of Integrated M. Sc. in Biotechnology, Anand 388001, Gujarat, India

Abstract


Objectives: A protocol was developed for plant regeneration using cotyledonary node of high-yielding indigenous pigeonpea [Cajanus cajan (L.) Millsp.] genotype GT 101. Methods: Induction of multiple shoots directly was completed from cotyledonary node as an explants on Murashige and Skoog’s (MS) medium supplemented with various concentrations and combination of 6-benzylaminopurine (BAP), kinetin and α-naphthalene acetic acid (NAA). Elongation of multiplied shoots was performed on MS medium supplemented with different combination of BAP and GA3. These well elongated plantlets were further transferred on MS medium supplemented with various concentrations of indole-3-butyric acid (IBA) for root induction. Regenerated plants were transferred to cocopeat:soil:vermiculite (2:1:2) for acclimation. Findings: The frequency of multiplication and number of multiple shoots from cotyledonary node explant were influenced on various types and concentrations of cytokinin. For multiple shoots induction, 3.0 mg/L BAP with 0.5 mg/L NAA was superior as compared to other combinations. The elongation of multiplied shoots was carried out on MS medium supplemented with 0.5 mg/L BAP and 0.5 mg/L GA3. The developed shoots were advanced to rooting on the medium supplemented with 0.5 mg/L IBA. They were subsequently grown in pots with 80% survival rate and these plants produced viable seeds. Improvement: The protocol for the production of in vitro multiple shoots with high frequency and their successive conversion to whole plants agreements potential for use in the improvement of protocol for development of transgenic in pigeonpea.

Keywords

Cajanus cajan (L.), Cotyledonary Node, GT101, In vitro regeneration, Pigeonpea.

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References


  • Salunkhe DK, Kadam SS, Chavan JK. Postharvest biotechnology of food legumes. CRC Press lnc. Boca Raton, Florida, USA. 1985. p. 29-52.
  • Faris DG, Singh U. Pigeonpea: Nutrition and products. International Crops Research for the Semi-Arid Tropics, Patancheru, Andhra Pradesh. 1990.
  • Lawn RJ, Troedson RJ. Pigeonpea: physiology of yield formation. In: Nene YL, Hall, SD, Sheila VK. The pigeonpea. 1990. p. 179-208.
  • Parekh M, Mahatma M, Kapadia C. In vitro regeneration of pigeonpea [Cajanus cajan (L.) Millsp.] genotype GT-102 using apical meristem. Journal of Cell and Tissue Research. 2014; 14(1): 4099-4103.
  • Nene YL, Sheila VK. Pigeonpea: geography and importance. In: Nene YL, Hall SD, Sheila VK (eds) The Pigeonpea. CAB International, Wallingford, UK, 1990. p. 1-14.
  • Prabhakaran M, Elumalai S, Ramganesh S, Prakasam V. High frequency induction of callus from seedling explants of Pigeonpea [Cajanus cajan (L.) Millsp.] for genetic transformation. Current Botany. 2011; 2(6): 15-17.
  • Sharma HC, Sharma KK, Crouch JH. Genetic transformation of crops for insect resistance: potential and limitation. Critical Reviews in Plant Sciences. 2004; 23(1): 47-72.
  • Geetha N, Venkatachalam P, Prakash V, Sita LG. High frequency induction of multiple shoots and plant regeneration from seedling explants of pigeonpea [Cajanus cajan (L.)]. Current Science. 1998; 75(10): 1036-1041.
  • Thu TT, Mai TTX, Dewaele E, Farsi S, Tadesse Y, Angenon G, Jacobs M. In vitro regeneration and transformation of pigeonpea [Cajanus cajan (L.) Millsp]. Molecular Breeding. 2003; 11(2): 159-168.
  • Srivastava J, Raghav PK. Effect of different gibberellic acid concentration on pigeonpea [Cajanus cajan (L.) millsp.] cv. MANAK (H77216) via cotyledonary node explants. International Journal of Advanced Biotechnology and Research. 2014; 5(2): 151-156.
  • Kumar VS, Lokesha R, Janagoudar BS, Muniswamy S. An Efficient Protocol for Whole Plant Regeneration via Auxiliary Bud Explants and Molecular Confirmation of pigeonpea [Cajanus cajan (L.) Millsp.] Regenerated Plants. Legume Genomics and Genetics. 2016; 7(4): 1-9.
  • Tyagi AP, Comai L, Byers B. Comparison of plant regeneration from root, shoot, and leaf explants in pigeonpea [Cajanus cajan (L.)] cultivars. SABRAO Journal of Breeding and Genetics. 2001; 33(2): 59-71.
  • Srinivasan T, Verma VK, Kirti PB. Efficient shoot regeneration in pigeonpea, [Cajanus cajan (L) Millisp.] using seedling petioles. Current Science. 2004; 86: 30-32.
  • Lawrence PK, Koundal KR. Agrobacterium tumefaciens-mediated transformation of pigeonpea [Cajanus cajan (L.) Millsp.] and molecular analysis of regenerated plants. Current Science. 2001; 80(11): 1428-1431.
  • Singh ND, Sahoo L, Jaiwal PK. In vitro shoot organogenesis and plant regeneration from cotyledonary node and leaf explants of pigeonpea [Cajanus cajan (L.) Millsp.]. Physiology and Molecular Biology of Plants. 2002; 8: 133-140.
  • Cheema HK, Bawa J. Clonal multiplication via multiple shoots in some legumes (Vigna unguiculata and Cajanus cajan). Acta Horticulturae. 1991; 289: 93-94.
  • George L, Eapen S. Organogenesis and embryogenesis from diverse explants in pigeonpea [Cajanus cajan (L.)]. Plant Cell Reports. 1994; 13(7): 417-420.
  • Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 1962; 15: 473-497.
  • Franklin G, Jeyachandran R, Ignacimuthu S. Factors affecting regeneration of pigeonpea [Cajanus cajan (L.) Millsp.] from mature embryonal axes. Plant Growth Regulation. 2000; 30(1): 31-36.
  • Ghosh G, Purohit A, Ganguly S, Chaudhuri RK, Chakraborti D. In vitro shoot grafting on rootstock: An effective tool for Agrobacterium-mediated transformation of pigeonpea [Cajanus cajan (L.) Millsp.]. Plant Biotechnology. 2014; 31:301-308.
  • Dayal S, Lavanya M, Devi P, Sharma KK. An efficient protocol for shoot regeneration and genetic transformation of pigeon pea [Cajanus cajan (L.) Millsp.] by using leaf explants. Plant Cell Report. 2003; 21: 1072–1079.
  • Villiers SD, Emongor Q, Njeri R, Gwata E, Hoisington D, Njagi I, Silim S, Sharma K. Evaluation of the shoot regeneration response in tissue culture of pigeon pea [Cajanus cajan (L.) Millsp.] varieties adapted to eastern and southern African Journal of Biotechnology. 2008; 7: 587–559.
  • Eapen S, Tivarekar S, George L. Thidiazuron-induced shoot regeneration in pigeonpea [Cajanus cajan (L.)]. Plant Cell, Tissue and Organ Culture. 1998; 53(3): 217-220.

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