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Phytochemical Analysis of Meizotropis pellita by FTIR and UV- VIS Spectrophotometer


  • School of Engineering and Technology, Ansal University, Gurgaon – 122003, Haryana, India
  • Shri Bankey Bihari Dental College Masuri, Ghaziabad - 201015, Uttar Pradesh, India


Objective: The present study investigates the characterization of the bioactive constituents showing antimicrobial activity present in leaf extract of Meizotropis pellita using UV-VIS and FTIR spectroscopy. Method: The methanolic extract of the leaves of Meizotropis pellita prepared by cold maceration method and analysed under ultraviolet and visible light. The scanning of the crude extract was done at the range from 190‐800 nm through Varian Cary -100 UV Visible spectrophotometer system and the characteristic peaks were detected. FTIR studies was done on a Jasco-4100 system, ranging 4000-400 cm-1 which revealed the characteristic peak values showing the absorption and functional groups present in leaf extract. Findings: The UV-VIS profile showed the peaks at 660, 340, 270, 235 and 210 nm with the absorption 0.085, 1.250, 2.605, 4.455 and 3.639. The result of FTIR spectra confirms the presence of alcohol, aldehydes carboxylic acid, aromatic and halogen compounds. So the present study provide evidences that leaves of Meizotropis pellita contain bioactive constituents which could be of interest for the development of new drug.


Bioactive Constitutents, FTIR spectrum, Meizotropis pellita, UV-VIS Spectrum.

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  • Ashis G. Herbal folk remedies of Bankura and Medinipur districts, West Bengal. Indian Journal of Traditional Knowledge. 2003; 2(4):393–6.
  • Parekh J, Chanda V. In vitro Antimicrobial activity and Phytochemical analysis of some Indian medicinal plants. Turkish J Biol. 2007; 31:53–8.
  • Liebler DC, Burr JA, Philips L, Ham A. Gas chromatography– mass spectrometry analysis of vitamin E and its oxidation products. Analytical Biochemistry. 1996; 236(1):27–34.
  • Aysal P, Ambrus AD, Lehotay SJ, Cannavan A. Validation of an efficient method for the determination of pesticide residues in fruits and vegetables using ethyl acetate for extraction. J Environ Sci Heal. 2007; 42:481–90.
  • Ibrahim M, Hameed AJ, Jalbout A. Molecular Spectroscopic Study of River Nile Sediment in the Greater Cairo Region. Applied Spectroscopy. 2008; 62(3):306–11.
  • Vijayarekha P, Sengottaiyan N. Phytochemical Evaluation, Antibacterial Activity and Bioactive Determination. Indian Journal of Science and Technology. 2016; 9(5):1–6.
  • Gunasekaran S. UV-VIS spectroscopic analysis of blood serum. Asian Journal of Microbiology Biotech and Environmental Science. 2003; 5(4):581–2.
  • Hashimoto A, Kameoka T. Applications of infrared spectroscopy to biochemical, food, and agricultural processes. Applied Spectroscopy Reviews. 2008; 43:416–51.
  • Hussain K, Ismail Z, Sadikun A, Ibrahim P. Evaluation of Metabolic Changes in Fruit of Piper armentosum in various Seasons by Metabolomics using Fourier Transform Infrared (FTIR) Spectroscopy. Intrenational Journal of Pharmaceutical and Clinical Research. 2009; 1(2):68–71.
  • Srinivasan PA, Arokiaraj A, Vijayarajan D. Isolation, spectral and optical analysis of fistulic acid – A phytochemical constituent of Cassia fistula Linn. Indian Journal of Science and Technology. 2011; 4(4):422–4.
  • Singh L, Nailwal TK, and Tiwari L. An in Vitro Approach for the Conservation of Meizotropis pellita: An Endangered and Endemic Plant. American Journal of plant Sciences. 2013; 4:1233–40.
  • Rani N, Sharma S, Sharma M. Antimicrobial activity of Meizotropis pellita (Patwa) against Gram’s positive and Grams negative bacteria. International Journal of Advanced Research. 2016; 4(4):1464–17.
  • Umeh EU, Oluma HOA, Igoli JO. Antibacterial screening of four local plants using an indicator- based microdilution technique. Afr J Trad. 2005; 2(3):238–43.
  • Kalakoti M, Kumar A. Phytochemical screening of leaf extract of Meizotropis pellita (Patwa): An Endangered Plant Species. International Journal of Advance Research. 2015; 3(4):361–5.
  • Silva GL, Lee L, Douglas K. Special problems with the extraction of plants. Natural Products Isolation.1998; 4:343–63.


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