Production of Cellulase from Pycnoporus sanguineus
Background/Objectives: Cellulase is an important enzyme used in the pulp and paper industries, detergent, textile and food. This work reports on the production of cellulose fromwhite-rot fungi Pycnoporussanguineus. Methods/Statistical Analysis: The fermentation of P.sanguineus was carried out using three different carbon sources that are cellulose, α-cellulose and carboxymethylcellulose to choose the most suitable carbon source for growth and enzymes production by P.sanguineus. The enzyme kinetics parameters (Km and Vmax) and the effect of metal ions on the cellulase enzyme activity were studied. The effects of some metal ions on the enzymes activity were also studied. Findings: The comparison of cellulase enzyme produced from both white rot fungi were done within 5 days of the fermentation time. The comparison was carried out in terms of growth rate of both fungus and the activity of FPase enzyme produced. Results showed that the FPase produced by P.sanguineus was six times higher than S.commune.The highest activity of FPase (18.00 U.mL-1), CMCase (7.28 U.mL-1) and EXOase (5.87 U.mL-1) was obtained on the fourth day of fermentation by P. sanguineuswhen α-cellulose was used as the carbon source. Then, α-cellulose was selected as the carbon source for the production of cellulase from P.sanguineus. The Vmax (1.54 µmol.h-1) and Km (0.5 mM) values of the cellulase enzyme using filter paper as the substrate were determined using Lineweaver-Burk plot. The addition of Mg2+ ions and Ca2+ ions increases the cellulase activity significantly. Application/Improvements: The addition of Mg2+ ions at concentration of 7mM and Ca2+ ions at 5mM concentration increases cellulase activity up to 19.2% and 16.2% respectively.
- Lakshmi AS, Narasimha G. Production of cellulase by fungal cultures isolated from forest litter soil. Journal of Research Article. 2012 Jan; 55(1):85–92.
- Sukumaran RK, Singhania RR, Pandey A. Microbial cellulasesproduction, applications and challenges. Journal of Scientific and Industrial Research. 2005 Nov; 64:832–44.
- Seyed SM, Fatemeh D, Farzaneh V. Recycling of Calotropis gigantea Waste fiber by enzymatic hydrolysis to produce bioethanol. Indian Journal of Science and Technology. 2016 Mar; 9(9):1–6.
- Musa MMT, Najeeb KNS, Nawal NA, Febri D, Wedad HA, Aidil AH, Sahaid KM, Mohtar WYW. Optimization of FPase activity using sorghum straw planted in Malaysia by Aspergillus terreus SUK-1 via solid substrate fermentation. Biotechnology. 2015; 14(1):23–8.
- Kang SW, Kim SW, Kimard K. Production of cellulases and xylanases by Aspergillusniger KKS. Journal of Microbiology and Biotechnology. 1994; 4(1):445–743.
- Wen Z, Liao W, Chen S. Production of cellulase by the mixed Aspergillusphoenicis on dairy manure. Journal of Applied Biochemistry and Biotechnology. 2005; 121:93–104.
- Shahriarinour M, Noor AWM, Rosfarizan M, Suhaimi, M. Effect of Medium composition and cultural condition on cellulase production by Aspergillusterrus. African Journal of Biotechnology. 2011 Jul; 10(38):7459–67.
- Zhang YHP, Hong J, Ye X. Cellulase assays. Journal of Molecular Biology. 2009; 581: 213–31.
- Hafiz MNI, Ishtiaq A, Muhammad AZ. Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichodermaviride. Advances in Bioscience and Biotechnology. 2011 Jun; 2(3):149–56.
- Duarte JB, Diego SMM, Villela SM, Morgado AF, Furigo A. A comparative study of mycelial growth and production of cinnabarin by different strains of Pycnoporussanguineus. BioFar: Revista de Biologia e Farmacia, Joao Pessoa. 2008; 2(2):1–5.
- Teoh YP, Mashitah MD. Cellulase production by Pycnoporussanguineus on oil palm residue through pretreatment and optimization study. Journal of Applied Sciences. 2010 Dec; 10(12):1036–43.
- Chen S, Wayman M. Novel inducers derived from starch for cellulase production by Trichodermareesei. Process Biochemistry. 1992; 27(6):327–34.
- Mandels M, Reese ET. Fungal cellulase and the microbial decomposition of cellulosic fabric. Journal of Bacteriology. 1999 Apr; 22(4):225–40.
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 3.0 License.