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Design and Production Control of Biocellulose from Acetobacter xylinum


  • Department of Chemical & Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia


Background/Objectives: This research aims to design a bioreactor control system for the production of biocellulose from fermented matured coconut water using Acetobacter xylinum. Methods/Statistical Analysis: The parameters for this research include pH, volume and concentration of glucose in the medium within a nine-day productivity of fermentation and incubated at static condition in 250 ml of conical flask. Findings: Based on the research, the productivity of biocellulose increases with the volume of the medium and the concentration of glucose in the medium decrease with time. The best pH value for producing biocellulose was pH 4.5 and pH decrease with time. Based on the stoichiometric equation, glucose was the limited reactant and degree of freedom is two. The controlled manipulated variable for this research was volume of the medium and pH level. A set of fermenter with controller produced higher productivity than a set of fermenter without controller. The average dry weight of biocellulose produced was 2.46 g at pH 4.5 and 30°C. Application/Improvements: Therefore, the fermentation process with the control system is more efficient with biocellulose production of 69.65% compare to that without controller system.


Acetobacter xylinum, Biocellulose, Control System, Fermenter, Matured Coconut Water.

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  • Shah J, Malcolm Brown R. Towards electronic paper displays made from microbial cellulose. Applied Microbiology Biotechnology. 2005 Jan; 66(4):352–55.
  • Yamanaka S, Iguchi M, Budhiono A. Bacterial cellulose-A masterpiece of nature’s arts. Journal of Material Science. 2000 Jan; 35(2):261–70.
  • Bogati D. Cellulose based biochemicals and their applications [Undergraduate]. Saimaa University of Applied Science; 2011 Aug.
  • Steinbuhel A. Bacterial cellulose. Biopolymers. Weinheim: Wiley-VCH; 2001.
  • De Iannino N, Couso R, Dankert M. Lipid-linked intermediates and the synthesis of acetan in acetobacter xylinum. Microbiology. 1988; 134:1731–36.
  • Tan T, Cheng L, Bhat R, Rusul G, Easa A. Composition, physicochemical properties and thermal inactivation kinetics of polyphenol oxidase and peroxidase from coconut (Cocos nucifera) water obtained from immature, mature and overly-mature coconut. Food Chemistry. 2014 Jan; 142:121–28.
  • Shuler M, Kargi F. Bioprocess engineering. Upper Saddle River, NJ: Prentice Hall; 2002. p. 169–73.
  • Rahman NA, Azmi MM, Zainuri MA, Laing S, Kasim N, Givajothi J. Application of process control in production of biocellulose: a case study. Applied Mechanics and Materials. 2015 Apr; 754–55:960–63.
  • Institut Penyelidikan dan Kemajuan Pertanian Malaysia (MARDI), Serdang: Selangor; 2014.
  • Kamarudin S, Sahaid KM, Sobri TM, Wan Mohtar WY, Radiah ABD, Norhasliza H. Different media formulation on biocellulose production by acetobacter xylinum (0416). Pertanika Journal of Science and Technology. 2013 Jan; 21(1):29.
  • Shoda M, Sugano Y. Recent advances in bacterial cellulose production. Biotechnology and Bioprocess Engineering. 2005; 10(1):1–8.
  • Jonas R, Farah L. Production and application of microbial cellulose. Polymer Degradation and Stability. 1998 Jan; 59(1):101–06
  • Czaja W, Krystynowicz A, Bielecki S, Brownjr R. Microbial cellulose—the natural power to heal wounds. Biomaterials. 2006 Jan; 27(2):145–51.
  • Rouessac F, Rouessac A. Chemical analysis. John Wiley & Sons, Ltd., Chichester; 2000.
  • Krieg N. Bergey’s manual of systematic bacteriology, Baltimore, MD: Williams & Wilkins; 1984.
  • Embuscado M, Marks J, BeMiller J. Bacterial cellulose. I. Factors affecting the production of cellulose by Acetobacter xylinum. Food Hydrocolloids. 1994 Oct; 8(5):407–18.
  • Hwang J, Yang Y, Hwang J, Pyun Y, Kim Y. Effects of pH and dissolved oxygen on cellulose production by Acetobacter xylinum BRC5 in agitated culture. Journal of Bioscience and Bioengineering. 1999; 88(2):183–88.
  • Aziz NM. Kajian terhadap kawalan pH dan medium dalam penghasilan bioselulosa bakteria oleh acetobacter xylinum 0046 (Study on controlled pH and medium in the production of bacterial biocellulose by acetobacter xylinum) [Undergraduate]. National University of Malaysia; 2012.
  • Mohammad S, Rahman NA, Khalil M, Abdullah SS. An overview of biocellulose production using acetobacter xylinum culture. Advances in Biological Research. 2014 Jan; 8(6):307–13.


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