Total views : 385
Purification of High Value Succinic Acid from Biomass Fermentation Broth Via Nanofiltration
Background/Objectives: Succinic acid purification from biomass fermentation experiences difficulty due to similar properties of acids presence. Thus purification work is conducted using nanofiltration (NF) membranes, and further characterizing its filtration performances. Methods/Statistical Analysis: The membranes used were NF1 and NF2 from AmforInc and NF270 from Filmtec. The investigated filtration performance parameters were different acids concentration and operating pressure of 5, 10, 20 and 50 g/L and 2, 5, 10 and 15 bar, respectively. Findings: From the results, NF270 showed the most efficient in purifying the succinic acid at 20 g/L and 50 g/L, which were 76% and 64.6% purification from 33% in the feed solution. Meanwhile for different pressure, NF2 shows the highest purification percentages which were 82% and 81% at the respective 5 and 10 bar followed by NF270 membrane at 68% and 67% at the respective 5 and 10 bar. The characterization of the membranes using DSC, FTIR, contact angle and SEM method shows that there was no fouling on the membranes observed after the filtration when there were no differences shown on the analysis results. This indicates that the range of pressure and concentration used in this study are suitable to perform the purification. Contact angle has found to influence the percentage of purification of each membrane where the less hydrophilic membrane shows the highest purification percentage. Application/Improvements: Thus NF270 and NF2 membranes were considered as the best candidate for purifying the succinic acid from fermentation broth.
Fermentation, Nanofiltration, Organic Acids, Polymer Membranes.
- Zeikus JG, Jain MK, Elankovan P. Biotechnology of succinic production and markets for derived industrial products. Applied Microbiology and Biotechnology. 1999; 51(5):545−52.
- Willke TH, Vorlop K-D. Industrial bioconversion of renewable resources as an alternative to conventional chemistry. Applied Microbiology and Biotechnology. 2004; 66(2):131−42.
- Sener A, Kadiata MM, Ladrie`re L, Malaisse WJ. Synergistic insulinotropic action of succinate, acetate, and glucose esters in islets from normal and diabetic rats. Endocrine. 1997; 7(2):151−55.
- Determination of Market Potential for Selected Platform Chemicals. http://www.weastra.com. Date accessed: 27/04/2015.
- Hutchings GJ. Vanadium phosphate: a new look at the active components of catalysts for the oxidation of butane to maleic anhydride. Journal of Materials Chemistry. 2004; 14:3385−95.
- Wan C, Li Y, Shahbazi A, Xiu S. Succinic acid production from cheese whey using Actinobacillus succinogenes. Applied Biochemistry and Biotechnology. 2008; 145(1):9−111.
- Cao Y, Zhang R, Sun C, Cheng T, Liu Y, Xian M. Fermentative Succinate Production: An Emerging Technology to Replace the Traditional Petrochemical Processes. BioMed Research International. 2013; 2013(11):1−12.
- Kang SH, Chang YK. Removal of organic acid salts from simulated fermentation broth containing succinate by nanofiltration. Journal of Membrane Science. 2005; 246(1):49−57.
- Song H, Huh YS, Lee SY, Hong WH, Hong YK. Recovery of succinic acid produced by fermentation of a metabolically engineered Mannheimia succiniciproducens strain. Journal of Biotechnology.2007; 132(4):445−52.
- Meynial-Salles I, Dorotyn S, Soucaille P. A new process for the continous production of succinic acid from glucose at high yield, titer, and productivity. Biotechnology Bioengineering. 2008; 99:129−35 . 11. Beauprez J, Mey MD, Soetaert W. Microbial succinic acid production: natural versus metabolic engineered producers. Process Biochemistry. 2010; 45(7):1103−14.
- Li Q, Wang D, Wu Y, Li WL, Zhang YJ, Xing JM. One step recovery of succinic acid from fermentation broths by crystallization. Separation and Purification Technology.2010; 72(3):294−300.
- Song H, Jang SH, Park JM, Lee SY. Modeling of batch fermentation kinetics for succinic acid production by Mannheimia succiniciproducens. Biochem Engineering Journal.2008; 40(1):107−15 .
- Huh YS, Jun Y-S, Hong YK, Song H, Lee SY, Hong WH. Effective purification of succinic acid from fermentation broth produced by Mannheimia succinicproducens. Process Biochemtry. 2006; 41:1461−65.
- Nam H-G, Park K-M, Lim SS, Mun S. Adsorption equilibria of succinic acid and lactic acid on amberchrom CG300C resin. Journal of Chemical and Engineering Data. 2011; 56(3):464−71.
- McKinlay JB, Vieille C, Zeikus JG. Prospects for a biobased succinate industry. Applied Microbiology and Biotechnology. 2007; 76:727−40.
- Staszak K, Wozniak MJ, Karas Z, Staniewski J, Prochaska K. Application of nanofiltration in the process of the separation of model fermentation broth components. Polish Journal of Chemical Technology. 2013; 15(4):1−4.
- Patel, R., et al., Overview of Industrial Filtration Technology and its Applications. Indian Journal of Science and Technology. 2010; 3(10):1121−27.
- Choi J-H, Fukushi K, Yamamoto K. A study on the removal of organic acids from wastewaters using nanofiltration membranes. Separation Purification Technology. 2008; 59(1):17−25.
- Bastrzyk J, Gryta M, Karakulski K. Fouling of nanofiltration membranes used for separation of fermented glycerol solutions. Chemical Papers. 2014; 68(6):65−757.
- Bellona C, Marts M, Drewes JE. The effect of organic membrane fouling on the properties and rejection characteristic of nanofiltration membranes. Separation and Purification Technology. 2010; 74(1):44−54.
- Luo J, Wan Y. Effect of highly concentrated salt on retention of organic solutes by nanofiltration polymeric membranes. Journal of Membrane Science. 2011; 372:145−53.
- Van der Burggen B, Schaep J, Wilms D, Vandecasteele C. Influence of molecular size, polarity and change on the retention of organis molecules by nanofiltration. Journal of Membrane Science.1999; 156:29−41.
- Isahak WN, Ismail ZAR, Yarmo MA. Highly Selective Glycerol Esterification over Silicotungstic Acid Nanoparticles on Ionic Liquid Catalyst. Industrial & Engineering Chemistry Research. 2014; 53(25):95−10285.
- Rabiller-Baudry M, Gouttefangeas F, Lannic JL, Rabiller P. Coupling of SEM-EDX and FTIR-ATR to (quantitatively) investigate organic fouling on porous organic composite membranes. In: Current Microscopy Contributions to Advances in Science and Technology; Mendez-Vilas A (ed.); Formatex.: 2012; p.1−11.
- Yuan Y, Lee TR. Contact Angle and Wetting Properties. In: Surface Science Techniques. Bracco G, Holst B (ed.); Springer-Verlag Berlin Heidelberg.: Houston; 2013.
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 3.0 License.