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Effect of Chromium and Zinc Accumulation on Antioxidant Enzymes during Phytoremediation in Amaranthus Viridis and Parthenium Hysterophorous


  • Department of Environmental Sciences, Maharishi Dayanand University, Rohtak 124001, Haryana,, India


Background/Objectives: The study investigate the variation in antioxidant response in experimental plant to overcome heavy metal stress and higher level of antioxidant enzymes may be associated with tolerance capacity of the weeds to defend the plant from oxidative damage. Methods/Statistical Analysis: Pot culture experiments were completed in greenhouse and the effect of chromium and zinc on antioxidants enzymes were investigated using UV- Spectrophotometer. A strong positive correlation were revealed in between concentration of heavy metals in various parts of plants and soil. Heavymetal stress enhanced the activity of CATalase (CAT) and Ascorbate PeroXidase (APX). Findings: Present study concluded that Parthenium hysterophorous and Amaranthus viridis has considerable ability to accumulate zinc and chromium and induced higher catalase and ascorbate peroxidase activity. Application/Improvement: The study describes a strategy and methodology that these two plants have ability to survive in stressed conditions and enhancing their environmental significance.


Antioxidant Enzymes, Heavy Metals, Reactive Oxygen Species and Phytoremediation.

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  • Khan S, Naz A, Asim M, Ahmad SA, Yousaf S, Muhammad S. Toxicity and Bioaccumulation of Heavy Metals in Spinach Seedlings Grown on Freshly Contaminated Soil, Pakistan Journal of Botany. 2013 Jan 1; 45(supplement 1):501−8.
  • Fitamo D, Leta S, Belay G, Lemma B, Olsson M. Phytoavailability of Heavy Metals and Metalloids in Soils Irrigated with Wastewater, Akaki, Ethiopia: a Greenhouse Study, Soil and Sediment Contamination: An International Journal. 2011 Oct 1; 20(7):745−66.
  • Jalali M, Dayani N. Heavy Metals (Cd, Cu, Ni, Pb, and Zn). Fractionation in River Sediments, Hamedan, Western Iran, Soil and Sediment Contamination: An International Journal. 2012 Aug 1; 21(6):756−67.
  • Uzu G, Sauvain JJ, Baeza-Squiban A, Riediker M, Sánchez Sandoval Hohl M, Val S, Tack K, Denys S, Pradere P, Dumat C. In Vitro Assessment of the Pulmonary Toxicity and Gastric Availability of Lead-Rich Particles from a Lead Recycling Plant, Environmental Science and Technology.2011 Aug 18; 45(18):7888−95.
  • Schreck E, Foucault Y, Sarret G, Sobanska S, Cécillon L, Castrec-Rouelle M, Uzu G, Dumat C. Metal and Metalloid Foliar Uptake by Various Plant Species Exposed to Atmospheric Industrial Fallout: Mechanisms Involved for Lead, Science of the Total Environment. 2012 Jun 15; 427:253−62.
  • Shahid M, Xiong T, Masood N, Leveque T, Quenea K, Austruy A, Foucault Y, Dumat C. Influence of Plant Species and Phosphorus Amendments on Metal Speciation and Bioavailability in a Smelter Impacted Soil: A Case Study of Food-Chain Contamination, Journal of Soils and Sediments. 2014 Apr 1; 14(4):655−65.
  • Pourrut B, Shahid M, Douay F, Dumat C, Pinelli E.Molecular Mechanisms Involved in Lead Uptake, Toxicity and Detoxification in Higher Plants. In: Heavy Metal Stress in Plants, Springer Berlin Heidelberg; 2013. p. 121−47.
  • Yang JE, Kim JS, Ok YS, Yoo KR. Mechanistic Evidence and Efficiency of the Cr (VI) Reduction in Water by Different Sources of Zerovalent Irons, Water Science and Technology.2007 Jan 1; 55(1-2):197−202.
  • Ok YS, Usman AR, Lee SS, El-Azeem SA, Choi B, Hashimoto Y, Yang JE. Effects of Rapeseed Residue on Lead and Cadmium Availability and Uptake by Rice Plants in Heavy Metal Contaminated Paddy Soil, Chemosphere.2011 Oct 31; 85(4):677−82.
  • Zheng W, Zheng F, Lian Y, Lin P. Accumulation and Dynamics of Cu, Pb, Zn and Mn Elements in Kandelia Candel (L.) Druce Mangrove Community of Jiulong River Estuary of Fujian, Acta Botanica Sinica. 1995 Dec; 38(3):227−33.
  • Murray P, Ge Y, Hendershot WH. Evaluating Three Trace Metal Contaminated Sites: A Field and Laboratory Investigation, Environmental Pollution. 2000 Jan 31; 107(1):127−35.
  • Chaney RL. Plant Uptake of Inorganic Waste Constituents.
  • Watanabe T, Broadley MR, Jansen S, White PJ, Takada J, Satake K, Takamatsu T, Tuah SJ, Osaki M. Evolutionary Control of Leaf Element Composition in Plants, New Phytologist. 2007 May 1; 174(3):516−23.
  • Behera SK, Singh MV, Singh KN, Todwal S. Distribution Variability of Total and Extractable Zinc in Cultivated Acid Soils of India and their Relationship with some Selected Soil Properties, Geoderma. 2011 May 15; 162(3):242−50.
  • Stephan CH, Courchesne F, Hendershot WH, McGrath SP, Chaudri AM, Sappin-Didier V, Sauvé S. Speciation of Zinc in Contaminated Soils, Environmental Pollution. 2008 Sep 30; 155(2):208−16.
  • Gonzalez-Fernandez O, Queralt I, Carvalho ML, Garcia G.Lead, Zinc, Arsenic and Copper Pollution in the Alluvial Plain of a Mining Wadi: The Beal Case (Cartagena–La Union Mining District, SE Spain), Water, Air, and Soil Pollution. 2011 Sep 1; 220(1-4):279−91.
  • Yi Y, Yang Z, Zhang S. Ecological Risk Assessment of Heavy Metals in Sediment and Human Health Risk Assessment of Heavy Metals in Fishes in the Middle and Lower Reaches of the Yangtze River Basin, Environmental Pollution. 2011 Oct 31; 159(10):2575−85.
  • Foy CD, Chaney RT, White MC. The Physiology of Metal Toxicity in Plants, Annual Review of Plant Physiology. 1978 Jun; 29(1):511−66.
  • Fosmire GJ. Zinc Toxicity. The American Journal of Clinical Nutrition, 1990 Feb 1; 51(2):225−7.
  • Nriagu J. Zinc Toxicity in Humans. Elsevier Publication; 2007, p. 1−7.
  • Hayat S, Khalique G, Irfan M, Wani AS, Tripathi BN, Ahmad A. Physiological Changes Induced by Chromium Stress in Plants: An Overview. Protoplasma. 2012 Jul 1; 249(3):599−611.
  • Ganesh KS, Baskaran L, Rajasekaran S, Sumathi K, Chidambaram AL, Sundaramoorthy P. Chromium Stress Induced Alterations in Biochemical and Enzyme Metabolism in Aquatic and Terrestrial Plants, Colloids and Surfaces B: Biointerfaces. 2008 Jun 1; 63(2):159−63.
  • Shanker AK, Cervantes C, Loza-Tavera H, Avudainayagam S. Chromium Toxicity in Plants, Environment international.2005 Jul 31; 31(5):739−53.
  • Park JH, Lamb D, Paneerselvam P, Choppala G, Bolan N, Chung JW. Role of Organic Amendments on Enhanced Bioremediation of Heavy Metal (Loid) Contaminated Soils, Journal of Hazardous Materials. 2011 Jan 30; 185(2):549−74.
  • Sun TR, Ottosen LM, Jensen PE, Kirkelund GM.Electrodialytic Remediation of Suspended Soil– Comparison of Two Different Soil Fractions, Journal of Hazardous Materials. 2012 Feb 15; 203:229−35.
  • Arshad M, Silvestre J, Pinelli E, Kallerhoff J, Kaemmerer M, Tarigo A, Shahid M, Guiresse M, Pradère P, Dumat C.A Field Study of Lead Phytoextraction by Various Scented Pelargonium Cultivars, Chemosphere. 2008 May 31; 71(11):2187−92.
  • Bhargava A, Carmona FF, Bhargava M, Srivastava S.Approaches for Enhanced Phytoextraction of Heavy Metals, Journal of Environmental Management. 2012 Aug 30; 105:103−20.
  • Pardo JM. Biotechnology of Water and Salinity Stress Tolerance, Current Opinion in Biotechnology. 2010 Apr 30; 21(2):185−96.
  • Mohammed A. Almalki. In-Vitro Antioxidant Properties of the Leaf Extract of Mimosa Pudica Linn, Indian Journal of Science and Technology. 2016 Apr, 9(13).
  • Lamb C, Dixon RA. The Oxidative Burst in Plant Disease Resistance, Annual Review of Plant Biology. 1997 Jun; 48(1):251−75.
  • Ashraf M, Ali Q. Relative Membrane Permeability and Activities of some Antioxidant Enzymes as the Key Determinants of Salt Tolerance in Canola (Brassica Napus L.), Environmental and Experimental Botany. 2008 May 31; 63(1):266−73.
  • Ajaj H. Ishakani, Nilesh H. Joshi, M. Ayaz, K. Sumara, Kailash H. Vadher. Antioxidant Potential, Polyphenols and Diphenol Content of Seaweed Available at Veraval Coast, Saurashtra Region - Gujarat, Indian Journal of Science and Technology. 2016 Jan; 9(4).
  • Polle A. Dissecting the Superoxide Dismutase-AscorbateGlutathione-Pathway in Chloroplasts by Metabolic Modeling, Computer Simulations as a Step towards Flux Analysis, Plant Physiology. 2001 May 1; 126(1):445−62.
  • Laloi C, Apel K, Danon A. Reactive Oxygen Signalling: The Latest News, Current Opinion in Plant Biology. 2004 Jun 30; 7(3):323−8.
  • Aebi H. Catalase in Vitro, Methods in Enzymology. 1984 Dec 31;105:121−6.
  • Nakano Y, Asada K. Hydrogen Peroxide is Scavenged by Ascorbate-Specific Peroxidase in Spinach Chloroplasts, Plant and Cell Physiology. 1981 Aug 1; 22(5):867−80.
  • Qureshi MI, Abdin MZ, Qadir S, Iqbal M. Lead-Induced Oxidative Stress and Metabolic Alterations in Cassia Angustifolia Vahl, Biologia Plantarum. 2007 Mar 1; 51(1):121−8.
  • Dat J, Vandenabeele S, Vranová E, Van Montagu M, Inzé D, Van Breusegem F. Dual action of the active oxygen species during plant stress responses. Cellular and Molecular Life Sciences CMLS. 2000 May 1;57(5):pp.779-95.
  • Noctor G, Foyer CH. Ascorbate and Glutathione: Keeping Active Oxygen Under Control, Annual Review of Plant Biology. 1998 Jun; 49(1):249−79.
  • Aravind P, Prasad MN. Zinc Alleviates Cadmium-Induced Oxidative Stress in Ceratophyllum Demersum L.: A Free Floating Freshwater Macrophyte, Plant Physiology and Biochemistry. 2003 Apr 1; 41(4):391−7.
  • Aravind P, Prasad MN. Modulation of Cadmium-Induced Oxidative Stress in Ceratophyllum Demersum by Zinc Involves Ascorbate–Glutathione Cycle and Glutathione Metabolism, Plant Physiology and Biochemistry. 2005 Feb 28; 43(2):107−16.
  • Kabata-Pendias A, Pendias H. Trace Elements in Soil and Plants. 3rd Edition. CRC Press. Boca Raton, London; 2001.p. 432.
  • Halliwell B, Gutteridge JM. Free Radicals in Biology and Medicine. Oxford University Press, USA; 2015 Jul 16.
  • Patel MJ, Patel JN, Subramanian RB. Effect of Cadmium on Growth and the Activity of H2O2 Scavenging Enzymes in Colocassia Esculentum, Plant and Soil. 2005 Jun 1; 273(12):183−8.
  • Kumar M, Kumari P, Gupta V, Anisha PA, Reddy CR, Jha B. Differential Responses to Cadmium Induced Oxidative Stress in Marine Macroalga Ulva Lactuca (Ulvales, Chlorophyta), Biometals. 2010 Apr 1; 23(2):315−25.
  • Dixit V, Pandey V, Shyam R. Differential Antioxidative Responses to Cadmium in Roots and Leaves of Pea (Pisum Sativum L. cv. Azad), Journal of Experimental Botany. 2001 May 1; 52(358):1101−9.
  • Tiedge M, Lortz S, Munday R, Lenzen S. Complementary Action of Antioxidant Enzymes in the Protection of Bioengineered Insulin-Producing RINm5F Cells Against the Toxicity of Reactive Oxygen Species, Diabetes. 1998 Oct 1; 47(10):1578−85.
  • Chelikani P, Fita I, Loewen PC. Diversity of Structures and Properties among Catalases, Cellular and Molecular Life Sciences CMLS. 2004 Jan 1; 61(2):192−208.


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