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Issue No:8 Vol.3 |
August-2010 |
ISSN: 0974-5645 |
| RESEARCH ARTICLES |
Viewers & PDF |
| 7. A new combinational statistical approach for cellulase optimization in Aspergillus nidulans. S. Anuradha Jabasingh and C. Valli Nachiyar. Indian J.Sci.Technol. Vol. 3, Issue 8, pp: 871-878. Domain site: http://www.indjst.org. |
 
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- Abstract
The enhancement of the cellulase activity of Aspergillus nidulans by combinational optimization technique was
investigated. The strain isolated from decayed, dry leaf of Ficus caricus was compared for the first time for its ability to
produce cellulolytic enzyme in submerged fermentation (SmF). The medium ingredients enhancing the cellulase
production were optimized by combinational statistical approach by one factor at a time methodology (OFAT), Plackett
Burmann methodology (PB) and response surface methodology (RSM). A four–factor–five-level central composite
design (CCD) was employed to determine the maximum activity of cellulase at optimum levels of carboxy methyl
cellulose (CMC), ammonium nitrate and potassium dihydrogen phosphate at varying pH values. The optimum
fermentation parameters were found to be 1.2 g/l CMC, 0.9 mg/l ammonium nitrate and 0.75 mg/l potassium
dihydrogen phosphate at pH 6. The optimization of medium by combinational statistical approach led to the fine tuning
of the cellulase production thereby enhancing the cellulase activity from 4.91 U/ml to 39.56 U/ml. The predicted results
were in agreement with the actual experimental values. The cellulase activity obtained with this strain may be one of
the best obtained in Aspergillus nidulans.
- Keywords: Aspergillus nidulans, cellulase activity, submerged fermentation, response surface methodology
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