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Issue No:12, Vol.3 |
Dec - 2010 |
ISSN: 0974-5645 |
| RESEARCH ARTICLES |
Viewers & PDF |
| .10. Cytotoxic effect of diphtheria toxin in mammalian cell lines. N. Sivananda, S. Ajesh, C. Manju, P. Sugapriya, M. G. Mukund Sudharshan, G. Maheswari, K. Priscilla, C. Palaniappan and B. Sekar. Indian J.Sci.Technol. Vol. 3, Issue 12, pp: 1177-1179. Domain site: http://www.indjst.org |
 
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- Abstract
Diphtheria toxin is an exotoxin secreted by Corynebacterium diphtheriae, the bacterial pathogen that causes
diphtheria. The present study was focused to find out the toxicological effect of diphtheria toxin in guinea pigs and cell
lines like Vero, MA104, HeLa, Murine neuroblastoma-2A, BHK-21, Rhabdomyosarcoma and L20B. Animals inoculated
with the toxin at different concentrations showed erythema around the site of inoculation after 48 h. When susceptible
cell lines inoculated with similar concentration of toxin showed similar morphological changes with certain minor
differences were observed. Diphtheria toxin has the capacity to inhibit protein synthesis in mammalian cell lines and
thus causing cell death. The study concluded that Vero, MA104 and Rhabdomyosarcoma cell lines could be used as
an alternative to in vivo assay for the toxicology study of diphtheria toxin and enables us free from ethical issues.
- Keywords: Diphtheria toxin, cell lines, erythema, guinea pigs.
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