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Issue No:12, Vol.3 |
Dec - 2010 |
ISSN: 0974-5645 |
| RESEARCH ARTICLES |
Viewers & PDF |
| .4. In vitro estrogenic potency of phytoestrogen-glycosides and some plant flavanoids. J. C. Kalita and S. R. Milligan. Indian J.Sci.Technol. Vol. 3, Issue 12, pp: 1142-1147. Domain site: http://www.indjst.org. |
 
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- Abstract
Many plants produce chemicals that mimic or interact with hormonal signals in animals. Their presence in human diet
is the object of many studies concerned with prevention of breast and prostate cancer, osteoporosis and other
hormone-dependent diseases. Isoflavones, daidzein and genistein occurring either free or bound in glycosides are the
main phytoestrogens in soya. The present study investigated the estrogenic activities of two isoflavones, two
glucosylated isoflavones, one isoflavandiol, two flavanoids and some other plant-derived phenolic compounds. The
bioassay used in this study was an estrogen responsive cell line Ishikawa Var-1. Besides, this study also used the
recombinant yeast cells bearing the human estrogen receptor for confirming the actual estrogenic nature of the plant
chemicals. There was a marked stimulation of alkaline phosphatase activity in Ishikawa cells and β-galactosidase
activity in yeast cells by glucosylated isoflavones, genistin and daidzin. Considering the potency of body’s endogenous
estrogen (17β-estradiol) as 100, in the Ishikawa assay the relative estrogenic potency of these compounds were found
to be genistein (0.11), genistin (0.06), daidzein (0.08) and daidzin (0.07). In the yeast cell assay, the relative estrogenic
potencies were genistein (0.1), genistin (0.01), daidzein (0.02) and daidzin (0.002). Estrogenic potency of equol was
almost equal (0.18 & 0.16) in both Ishikawa and yeast cell assays, respectively. While kaempferol and resveratrol
were weakly estrogenic, quercetin, rutin, catechin, hesperetin and luteolin did not show any estrogenic activity.
- Keywords: Phytoestrogen, flavanoids, estrogenic activity, glycosides.
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