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Issue No:7, Vol.3 |
July - 2010 |
ISSN: 0974-5645 |
| RESEARCH ARTICLES |
Viewers & PDF |
| 16. In vitro propagation of Bacopa monnieri L. - a multipurpose medicinal plant. M. Vijayakumar, R. Vijayakumar and R. Stephen. Indian J.Sci.Technol. Vol. 3, Issue 7, pp: 782-787. Domain site: http://www.indjst.org. |
 
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- Abstract
We report the micropropagation of Bacopa monnieri L. a medicinal plant from Shervarayan Hills, Salem, Tamil Nadu,
India. Effect of auxins (IAA, NAA & 2, 4-D) and cytokinins (BA, KIN & TDZ) on shoot induction and IAA and TDZ on root
induction were assessed. Callus induction on Murashige and Skoog’s medium (MS) supplemented with NAA, 2,4-D
and TDZ at various combinations/concentrations were also investigated. The auxiliary buds formed from both leaf and
nodal explants on hormone free MS medium within 9 d. Growth hormones at different combinations brought out
remarkable variations in shoot, root and callus induction. Rooted plantlets were transferred to 10 different soil mixtures
with various ratio/combinations of biological and natural fertilizers such as humus rich soil alone (control), soil with
vesicular arbuscular mycorrhizae (VAM) (10:1 & 10:2) soil with vermicompost (4:1 & 4:2) soil with farmyard manure
(4:1 & 4:2) soil with flyash (1:1 & 1:2) and soil with agropeat (4:1 & 4:2) in sterile polythene bags. When the plantlets
were transplanted to the actual field, this pre-adaptation increased their survival as much as 80-90% (observed for 45
days).
- Keywords: Bacopa monnieri L., growth hormones, auxiliary shoot proliferation, transplantation.
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