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Issue No:5, Vol.3 |
May - 2010 |
ISSN: 0974-5645 |
| RESEARCH ARTICLES |
Viewers & PDF |
| 1. Identification of some wild Vicia species using electrophoretic analysis of seed proteins and amino acids composition. M.M. Sakr, S.M.H. Gowayed, W.M. Hassan and O.A. Almghraby (2010) Indian J.Sci.Technol. Vol. 3, Issue 5, pp: 490-498. Domain site: http://www.indjst.org |
 
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- Abstract
We studied protein variation among the Egyptian species of wild Vicia that may be of taxonomical importance. Seeds
of five wild Vicia species (Vicia sativa subsp. amphicarpa, V. articulata, V. ervilia, V. narbonensis and V. villosa) were
collected from their natural habitats in Egypt. Protein, albumin and globulin electrophoresis was carried out on the
seeds. Moreover, protein content and its amino acids, albumin and globulin were determined. Total bands and their
distribution and similarity percentage differed according to the studied Vicia species and protein type. Generally, the
highest values of total bands for total protein, albumin and globulin were observed in V. sativa subsp. amphicarpa.
Amount of albumin, globulin, total protein, albumin/total protein, globulin/ total protein and amino acids varied
according to the studied Vicia species. In general, the highest values of determined amino acids, albumin/total protein
and globulin/ total protein ratio were recorded in V. articulata. Furthermore, numerous taxonomical markers were
observed for each studied Vicia species on basis of total protein, albumin and globulin electrophoresis. These identity
electrophoregram of the studied species can be used as a tool for identifying the genetic diversity in Vicia species.
These results support the validity of seed protein as a powerful tool for Vicia species identification and clarifying
taxonomic relationships
- Keywords: Vicia, electrophoresis, seed proteins, dendogram, amino acids, legume, gentic diversity, taxonomy
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